• Brachybacterium muris

    © LG1
  • Brachybacterium muris, 1000 x Gram

    © LG1

Kurzinformation:Brachybacterium

Arten Brachybacterium alimentarium; B. conglomeratum; B. faecium; B fresconis; B. muris ; B. nesterenkovii; B. paraconglomeratum; B. rhamnosum; B. sacelli; B. tyrofermentans.

Bekannte Infektionen Wurde bisher nur als Umweltkeim beschrieben. Wir konnten Brachybacterium muris aus einem Gehörgang-Abstrich bei Otitis media mit Hämophilus influenzae isolieren.

Empfindlichkeiten u. Therapie keine bekannten Infektionen

Systematik Dermabacteraceae (Nicht sporenbildende, aerob wachsende, grampositive Stäbchen.)
The family Dermabacteraceae (Stackebrandt et al., 1997) was established on the basis of 16S rDNA sequence data and circumscribed by a set of specific signature nucleotides. It contains the genera Dermabacter (Jones and Collins, 1988) and Brachybacterium (Collins et al., 1988).

Literatur BUCZOLITS (S.), SCHUMANN (P.), WEIDLER (G.), RADAX (C.) et BUSSE (H.J.) : Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. Int. J. Syst. Evol. Microbiol., 2003, 53, 1955-1960.
COLLINS (M.D.), BROWN (J.) et JONES (D.) : Brachybacterium faecium gen. nov., sp. nov., a coryneform bacterium from poultry deep litter. Int. J. Syst. Bacteriol., 1988, 38, 45-48.
GVOZDYAK (O.R.), NOGINA (T.M.) et SCHUMANN (P.) : Taxonomic study of the genus Brachybacterium: Brachybacterium nesterenkovii sp. nov. Int. J. Syst. Bacteriol., 1992, 42, 74-78.
HEYRMAN (J.), BALCAEN (A.), DE VOS (P.), SCHUMANN (P.) et SWINGS (J.) : Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria). Int. J. Syst. Evol. Microbiol., 2002, 52, 1641-1646.
TAKEUCHI (M.), FANG (C.X.) et YOKOTA (A.) : Taxonomic study of the genus Brachybacterium: proposal of Brachybacterium conglomeratum sp. nov., nom. rev., Brachybacterium paraconglomeratum sp. nov., and Brachybacterium rhamnosum sp. nov. Int. J. Syst. Bacteriol., 1995, 45, 160-168.
SCHUBERT (K.), LUDWIG (W.), SPRINGER (N.), KROPPENSTEDT (R.M.), ACCOLAS (J.P.) et FIEDLER (F.) : Two coryneform bacteria isolated from the surface of French Gruyère and Beaufort cheeses are new species of the genus Brachybacterium: Brachybacterium alimentarium sp. nov. and Brachybacterium tyrofermentans sp. nov. Int. J. Syst. Bacteriol., 1996, 46, 81-87


The Journal of hospital infection, Vol. 57 (1), p: 52-8 Erscheinungsjahr 2004,
Abstract: Universal or 'broad-range' eubacterial polymerase chain reaction (PCR) was performed on 53 isolates from environmental water-associated sites in a haematology unit [Formula: see text] and the outer surfaces of cleaning lotion containers sited throughout a tertiary referral hospital [Formula: see text] 16 S rDNA PCR was performed using two sets of universal primers, including the novel reverse primer, XB4, to generate a composite amplicon of 1068 bp, which was sequenced to obtain each isolate's identity. Sequence analysis was able to identify 51 isolates. Most (75% from the haematology unit and 81% from cleaner containers) were Gram-positive. Nine different genera were identified from the haematology unit and 13 from the cleaning lotion containers. This study provides the first reports of Terrabacter spp. and Brachybacterium paraconglomeratum isolated from a hospital environment. As unusual and difficult-to-identify environmental organisms are unlikely to be clinically significant, and molecular identification is costly and labour-intensive, we recommend that molecular methods are only used as an adjunct to first-line phenotypic identification schemes where a definitive identification is required. Where molecular identification methods are justified, partial 16 S rDNA PCR and sequencing employing the novel universal primer XB4, is a valuable and reliable technique.

Spezielles

Brachybacterium sollten kurze (Brachys = kurz) grampositive Stäbchen sein, im Grampräparat sind es jedoch mehrheitlich runde Kokken. In den konventionellen, kommerziellen Identifikationssystemen wird Brachybacterium muris als Micrococcus spp. identifiziert.
Mit der 16S rRNA-PCR kann der Keim gut identifiziert werden.